PART Ⅰ INTRoDUCTION
CHAPTER 1 INTRoDUCl’ION TO MoLECULAR EPIDEMIOLoGY
1—1 Basic concept of molecular epidemiology
1—2 Why molecular epidemiology
1—3 Comparisons between classical and molecular methods in epidemiology
1—4 Working approaches of molecular epidemiology
1—5 About the book
References and suggested readings
PART Ⅱ MoLECULAR TECHNIQUES USED IN EPIDEMIOLOGY
CHAPTER 2 MOLECULAR MARKERS
2—1 Protein—based marker：Allozymes
2—2 DNA band—based markers
2—3 DNA sequence
References and suggested readings
CltAPTER 3 PCR—BASED TECHNIQUES
3—1 Classical PCR
3—2 Allele—specific PCR
3—3 Nested PCR
3—4 Multiplex PCR
References and suggested readings
CHAPTER 4 QUANTITATIVE REAL‘TIME PCR
4—1 Introduction
4—2 Basic real—time PCR methods
4—3 Primer and probe design for real—time PCR
4—4 Result analysis
4—5 Applications of real—time PCR
References and suggested readings
CHAmR 5 PRIMER I）ESIGN
5—1 Some basic concepts for primer design
5—2 Computer programs used for primer design
5—3 An example of primer design using a computer program
5—4 Degenerated PCR primers
PART Ⅲ MoLECULAR EPIDEMIOLOGY oF PLANT DISEASES
CHAPTER 6 PATHoGEN IDENTIFICATIoN AND DISEASE DIAGNoSIS
6—1 Introduction
6—2 Use of species—specific genes or DNA regions for developing PCR primers
6—3 Use of anonymous unique DNA regions for developing PCR primers
6—4 PCR inhibitors
Summary
References and suggested readings
CHAPTER 7 FUNGAL PATHoGEN REPRoDUCTIVE MoDES
7—1 Frequencies of genotypes
7—2 Deviation from Hardy—Weinberg expectations
7—3 Parsimony Tree Length Permutation（PTLPT）
7—4 Index of association（IA）
References and suggested readings
CHAPTER 8 DETEMINATIoN AND QUANTIFICATION OF INITIAL INOCULUM OF EPIDEMICS
8—1 Introduction
8—2 Molecular methods for quantification of initial inoculum
8—3 Examples of quantification of initial inoculum using molecular methods
Remarkable conclusion
References and suggested readings
CHPTER 9 TEMpORAL AND SPATIAL DEVELOPMENT oF PATHoGEN POPULATIoNS
9—1 Introduction
9—2 Molecular approaches
9—3 Monitoring pathogen population structures
9—4 Studying spatial—temporal dynamics of pathogen populations
9—5 Providing information needed for disease management
Summary
References and suggested readings
CHAPTER 10 PATHoGEN LONG—DISTANCE DIsPERSAL AND MIGRATION
10—1 Introduction
10—2 Pathogen lon9—distance dispersal and disease epidemics
10—3 Traditional methods to determine or infer pathogen lon9—distance dispersal
10—4 Molecular methods to study pathogen lon9—distance dispersal
10—5 Examples
Summary
……
PART IV METHODOLOGY OF DATA ANLYSIS
INDEX
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