生物技术概论（双语教材）
目 录内容简介
Preface
Part I Gene Cloning
Chapter 1 Introduction to Gene Cloning
1.1 Brief Overview
1.2 Historical Events
1.3 Significance and Alertness
Chapter 2 Gene and Genome
2.1 Discovery of Hereditary Factor
2.2 Basic Knowledge of Gene
2.3 Sequence Signals Involved with Gene Expression
2.4 About Genomic and Metagenomic Studies
Chapter 3 Basic Techniques for Experiments with DNA
3 1 Enzymatic Tools for Gene Manipulation
3.20ligonucleotide Tools
3.3 DNA Purification
3.4 Electrophoresis
3.5 In Vitro Synthesis of DNA
3.6 DNA Sequencing
3.7 Macromolecular Hybridization and Blotting
3.8 PCR Techniques
Chapter 4 Cloning Procedure
4.1 Basic Procedure of Cloning
4 2 Selection of Cloning Vecto
4.3 Preparation of Donor DNA
4.4 Joining of Donor and Vector by Ligation
4.5 Selection Host Cell
4.6 Introduction of Recombinant DNA into Host Cells
4.7 "Selection, Screening and Subcloning
4.8 Derivative Technology Based on Cloning
Chapter 5 Application and Impacts
5.1 Initiation of Recombinant DNA Industry
5.2 General Aspects of Application
5.3 Contribution and Impact
Part II Cell Engineering
Chapter 6 Introduction on Cell Engineering
6.1 Brief Overview
6.2 Essentials of Cell Engineering
6.3 Progress Clues
Chapter 7 Cell Fusion and Hybridization
7.1 General Aspects
7.2 Protoplast Technique
7.3 Basic Methods of Cell Fusion
7.4 Fusant Screening
7.5 Application of Cell Fusion
Chapter 8 Plant Tissue and Cell Culture
8.1 Plant Tissue Culture System
8.2 Gene Tra fer to Plants
8.3 Artificial Seeds and Virusfree Plants
Chapter 9 Animal Tissue and Cell Culture
9.1 Establishment of Animal Tissue Culture System
9.2 Gene Tra fer to Animal Cell
9.3 Animal Hybrids and Hybridoma
9.4 Approaches of Stem Cell Research
Chapter 10 Application Examples from Cell Engineering
10.1 Bioproducts from Cell Engineering
10.2 Breeding of New Agronomic Varieties
10.3 Tra genic Animals as Models for Medical Research
Part III Enzyme Technology
Chapter 11 Fundamental Nature of Enzyme
11.1 Definition, Basic Nature and Classification of Enzyme
11.2 Determination of Enzyme Activities
11.3 Facto Affecting Enzyme Activity
11.4 Resources of Enzyme
11.5 Uses of Enzyme
Chapter 12 Enzyme Biosynthesis and Products
12.1 BiDresources of Enzyme
12.2 About Enzyme Synthesis
12.3 Enzyme Fermentation and Products
Chapter 13 Enzyme Recovery& Purification
13.1 Pretreatment of Crude Enzyme Sample
13.2 Removing of Impurities and, Sample Concentration
13.3 Chromatographic Separation
13.4 Polishing of Enzyme
13.5 Rational Formulation of Final Enzyme Products
Chapter 14 Enzymatic Conve ion
14.1 Enzymatic Conve ion
14.2 Immobilization of Enzyme and Cells
14.3 Enzymatic conve ion in nonaqueous Phase
Chapter 15 New Approaches of Enzyme Technology
15.1 Modification on Enzymes
15.2 Enzyme Carrie for Immobilization
15.3 Enzyme Inhibito
15.4 Ribozyme （Nonprotein Enzymes）
Part IV Industrial Fermentation
Chapter 16 Principles of Fermentation
16.1 Fermentation Principles
16.2 Fermentation Products and Application
16.3 Benefits from the Fermentation Industry
Chapter 17 Industrial Microorganisms
17.1 Types and Characte of Industrial Microorganisms
17.2 Breeding of Industrial Strai
17.3 Industrial Microbial Growth and Culture Media
17.4 Inoculation of Industrial Strai
17.5 Preservation of Industrial Strai
Chapter 18 Microbial Metabolism and Fermentation Kinetics
18.1 Metabolism Related toBasic Pathway
18.2 Metabolic Regulation and Control
18.3 Stoichiometry of Fermentation Process
18.4 Fermentation Kinetics
Chapter 19 Bioreacto and Auxiliary Equipments
19.1 Structure and Function of Bioreacto
19.2 Auxiliary Equipment
19.3 Probes and Se o
Chapter 20 Fermentation DownStream Technology
20.1 General Aspects
20.2 Harvest and Pretreatment
20.3 Isolation and Purification
20.4 Product Polishing
20.5 Waste Treatment and Cleaner Production
Chapter 21 Progress in Fermentation Engineering
21.1 Approaches of Upstream Technology
21.2 Process Optima
21.3 Development of Dow tream Technology
Appendix
Terms Tra lation
Related Nobel Laureates
Further Reading
后记
Part I Gene Cloning
Chapter 1 Introduction to Gene Cloning
1.1 Brief Overview
1.2 Historical Events
1.3 Significance and Alertness
Chapter 2 Gene and Genome
2.1 Discovery of Hereditary Factor
2.2 Basic Knowledge of Gene
2.3 Sequence Signals Involved with Gene Expression
2.4 About Genomic and Metagenomic Studies
Chapter 3 Basic Techniques for Experiments with DNA
3 1 Enzymatic Tools for Gene Manipulation
3.20ligonucleotide Tools
3.3 DNA Purification
3.4 Electrophoresis
3.5 In Vitro Synthesis of DNA
3.6 DNA Sequencing
3.7 Macromolecular Hybridization and Blotting
3.8 PCR Techniques
Chapter 4 Cloning Procedure
4.1 Basic Procedure of Cloning
4 2 Selection of Cloning Vecto
4.3 Preparation of Donor DNA
4.4 Joining of Donor and Vector by Ligation
4.5 Selection Host Cell
4.6 Introduction of Recombinant DNA into Host Cells
4.7 "Selection, Screening and Subcloning
4.8 Derivative Technology Based on Cloning
Chapter 5 Application and Impacts
5.1 Initiation of Recombinant DNA Industry
5.2 General Aspects of Application
5.3 Contribution and Impact
Part II Cell Engineering
Chapter 6 Introduction on Cell Engineering
6.1 Brief Overview
6.2 Essentials of Cell Engineering
6.3 Progress Clues
Chapter 7 Cell Fusion and Hybridization
7.1 General Aspects
7.2 Protoplast Technique
7.3 Basic Methods of Cell Fusion
7.4 Fusant Screening
7.5 Application of Cell Fusion
Chapter 8 Plant Tissue and Cell Culture
8.1 Plant Tissue Culture System
8.2 Gene Tra fer to Plants
8.3 Artificial Seeds and Virusfree Plants
Chapter 9 Animal Tissue and Cell Culture
9.1 Establishment of Animal Tissue Culture System
9.2 Gene Tra fer to Animal Cell
9.3 Animal Hybrids and Hybridoma
9.4 Approaches of Stem Cell Research
Chapter 10 Application Examples from Cell Engineering
10.1 Bioproducts from Cell Engineering
10.2 Breeding of New Agronomic Varieties
10.3 Tra genic Animals as Models for Medical Research
Part III Enzyme Technology
Chapter 11 Fundamental Nature of Enzyme
11.1 Definition, Basic Nature and Classification of Enzyme
11.2 Determination of Enzyme Activities
11.3 Facto Affecting Enzyme Activity
11.4 Resources of Enzyme
11.5 Uses of Enzyme
Chapter 12 Enzyme Biosynthesis and Products
12.1 BiDresources of Enzyme
12.2 About Enzyme Synthesis
12.3 Enzyme Fermentation and Products
Chapter 13 Enzyme Recovery& Purification
13.1 Pretreatment of Crude Enzyme Sample
13.2 Removing of Impurities and, Sample Concentration
13.3 Chromatographic Separation
13.4 Polishing of Enzyme
13.5 Rational Formulation of Final Enzyme Products
Chapter 14 Enzymatic Conve ion
14.1 Enzymatic Conve ion
14.2 Immobilization of Enzyme and Cells
14.3 Enzymatic conve ion in nonaqueous Phase
Chapter 15 New Approaches of Enzyme Technology
15.1 Modification on Enzymes
15.2 Enzyme Carrie for Immobilization
15.3 Enzyme Inhibito
15.4 Ribozyme （Nonprotein Enzymes）
Part IV Industrial Fermentation
Chapter 16 Principles of Fermentation
16.1 Fermentation Principles
16.2 Fermentation Products and Application
16.3 Benefits from the Fermentation Industry
Chapter 17 Industrial Microorganisms
17.1 Types and Characte of Industrial Microorganisms
17.2 Breeding of Industrial Strai
17.3 Industrial Microbial Growth and Culture Media
17.4 Inoculation of Industrial Strai
17.5 Preservation of Industrial Strai
Chapter 18 Microbial Metabolism and Fermentation Kinetics
18.1 Metabolism Related toBasic Pathway
18.2 Metabolic Regulation and Control
18.3 Stoichiometry of Fermentation Process
18.4 Fermentation Kinetics
Chapter 19 Bioreacto and Auxiliary Equipments
19.1 Structure and Function of Bioreacto
19.2 Auxiliary Equipment
19.3 Probes and Se o
Chapter 20 Fermentation DownStream Technology
20.1 General Aspects
20.2 Harvest and Pretreatment
20.3 Isolation and Purification
20.4 Product Polishing
20.5 Waste Treatment and Cleaner Production
Chapter 21 Progress in Fermentation Engineering
21.1 Approaches of Upstream Technology
21.2 Process Optima
21.3 Development of Dow tream Technology
Appendix
Terms Tra lation
Related Nobel Laureates
Further Reading
后记
目 录内容简介
《双语教材：生物技术概论》参考国内外学科进展，结合多年教学实践过程中积累的教学资源，针对工科为主的生物工程学科特点，选择贴近生物技术产业的科技基础内容，构成了由基因工程、细胞工程、酶工程，发酵工程四个板块（篇）组成的内容体系，每篇再以章、节、小节对有关内容进行阐述。讲义中还配有图表、附录、题库、电子教材、网站等有助于学生理解和应用的信息内容和形式；形成了立体化的教学资源。
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